Analysis Note
ControlStaurosporine treated Jurkat cells.
Included Negative Control: Catalog # 16-240, Alexa Fluor® 488-conjugated Normal Mouse IgG.
Application
Research CategoryApoptosis & Cancer
Detect Phosphatidylserine using this Anti-Phosphatidylserine Antibody, clone 1H6, Alexa Fluor 488 validated for use in FC.
Research Sub CategoryApoptosis - Additional
Disclaimer
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Significance:
Anti-Phosphatidylserine may be used to detect translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer cell membrane leaflet; it provides an alternative to Annexin V for quantitation of apoptosis.
Phosphatidylserine, or PS, is a naturally occurring, phospholipid nutrient. PS is essential to the functioning of all the cells of the body, but is most concentrated in the brain. Its relative abundance in this organ reflects its proven involvement in an assortment of nerve cell functions, including nerve transmitter release and synaptic activity. Clinical studies have suggested that PS can support brain functions that tend to decline with age.
Immunogen
Liposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
ALEXA FLUOR is a registered trademark of Life Technologies
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Protein G Purified
Purified mouse monoclonal IgG in buffer containing PBS containing 1% BSA, 0.05% Tween, 0.05% sodium azide. Liquid at 4°C.
Quality
Flow Cytometry: 0.2 µg of this antibody detected Phosphatidylserine in fixed Jurkat cells (see page 2).
Apoptosis Assay: Time course for induction of apoptosis in Jurkat cells by staurosporine, measured using either an Anti-Phosphatidylserine, clone 1H6, Alexa Fluor® 488 conjugate or an Annexin V FITC conjugate.
Specificity
Recognizes phosphatidylserine (PS) in cell membranes.
Storage and Stability
Stable for 1 year at from date of receipt.
This product has met the following criteria to qualify for the following awards: